Regulatory

Part:BBa_K165038:Design

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-29)


Gli1 binding sites + ADH1 constitutive yeast promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 122
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 315
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 119
    Illegal BsaI site found at 965


Design Notes

-


Source

Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.

References